Lucas Oil Stadium - March 8, 2025
| Position | # | Rider | Interval | Best Time |
|---|---|---|---|---|
| 1 | 27 | Malcolm Stewart | 48.76 | |
| 2 | 94 | Ken Roczen | 0.293 | 49.05 |
| 3 | 4 | Chase Sexton | 1.075 | 49.84 |
| 4 | 2 | Cooper Webb | 1.090 | 49.85 |
| 5 | 14 | Dylan Ferrandis | 1.584 | 50.35 |
| 6 | 51 | Justin Barcia | 1.639 | 50.40 |
| 7 | 7 | Aaron Plessinger | 1.806 | 50.57 |
| 8 | 32 | Justin Cooper | 1.957 | 50.72 |
| 9 | 21 | Jason Anderson | 2.149 | 50.91 |
| 10 | 17 | Joey Savatgy | 2.609 | 51.37 |
| 11 | 57 | Benny Bloss | 2.654 | 51.42 |
| 12 | 12 | Shane McElrath | 2.664 | 51.43 |
| 13 | 46 | Justin Hill | 3.214 | 51.98 |
| 14 | 77 | Jerry Robin | 3.605 | 52.37 |
| 15 | 45 | Colt Nichols | 3.706 | 52.47 |
| 16 | 49 | Mitchell Oldenburg | 3.996 | 52.76 |
| 17 | 72 | Anthony Rodriguez | 4.330 | 53.09 |
| 18 | 146 | Kevin Moranz | 4.709 | 53.47 |
| 19 | 122 | Justin Starling | 4.806 | 53.57 |
| 20 | 11 | Kyle Chisholm | 5.040 | 53.80 |
| 21 | 86 | Mitchell Harrison | 5.741 | 54.50 |
| 22 | 33 | Fredrik Noren | 6.054 | 54.82 |