Daytona International Speedway - March 1, 2025
| Position | # | Rider | Interval | Best Time |
|---|---|---|---|---|
| 1 | 32 | Justin Cooper | 1:20.66 | |
| 2 | 2 | Cooper Webb | 0.636 | 1:21.29 |
| 3 | 4 | Chase Sexton | 0.861 | 1:21.52 |
| 4 | 46 | Justin Hill | 1.229 | 1:21.88 |
| 5 | 27 | Malcolm Stewart | 1.252 | 1:21.91 |
| 6 | 51 | Justin Barcia | 2.751 | 1:23.41 |
| 7 | 7 | Aaron Plessinger | 2.770 | 1:23.43 |
| 8 | 49 | Mitchell Oldenburg | 3.452 | 1:24.11 |
| 9 | 17 | Joey Savatgy | 3.528 | 1:24.18 |
| 10 | 14 | Dylan Ferrandis | 3.619 | 1:24.27 |
| 11 | 21 | Jason Anderson | 3.850 | 1:24.51 |
| 12 | 12 | Shane McElrath | 4.534 | 1:25.19 |
| 13 | 94 | Ken Roczen | 4.577 | 1:25.23 |
| 14 | 86 | Mitchell Harrison | 7.651 | 1:28.31 |
| 15 | 33 | Fredrik Noren | 7.664 | 1:28.32 |
| 16 | 45 | Colt Nichols | 7.745 | 1:28.40 |
| 17 | 77 | Jerry Robin | 8.194 | 1:28.85 |
| 18 | 146 | Kevin Moranz | 8.297 | 1:28.95 |
| 19 | 72 | Anthony Rodriguez | 10.800 | 1:31.46 |
| 20 | 122 | Justin Starling | 16.239 | 1:36.90 |
| 21 | 11 | Kyle Chisholm | 19.278 | 1:39.93 |
| 22 | 57 | Benny Bloss | 20.867 | 1:41.52 |