Daytona International Speedway - March 1, 2025
| Position | # | Rider | Interval | Best Time |
|---|---|---|---|---|
| 1 | 4 | Chase Sexton | 1:18.35 | |
| 2 | 32 | Justin Cooper | 2.299 | 1:20.65 |
| 3 | 46 | Justin Hill | 2.407 | 1:20.75 |
| 4 | 2 | Cooper Webb | 2.441 | 1:20.79 |
| 5 | 94 | Ken Roczen | 2.818 | 1:21.16 |
| 6 | 27 | Malcolm Stewart | 2.896 | 1:21.24 |
| 7 | 21 | Jason Anderson | 3.070 | 1:21.42 |
| 8 | 51 | Justin Barcia | 3.774 | 1:22.12 |
| 9 | 17 | Joey Savatgy | 3.936 | 1:22.28 |
| 10 | 7 | Aaron Plessinger | 3.965 | 1:22.31 |
| 11 | 14 | Dylan Ferrandis | 4.309 | 1:22.66 |
| 12 | 49 | Mitchell Oldenburg | 4.789 | 1:23.14 |
| 13 | 12 | Shane McElrath | 5.383 | 1:23.73 |
| 14 | 11 | Kyle Chisholm | 6.665 | 1:25.01 |
| 15 | 45 | Colt Nichols | 6.919 | 1:25.27 |
| 16 | 77 | Jerry Robin | 6.947 | 1:25.29 |
| 17 | 72 | Anthony Rodriguez | 7.730 | 1:26.08 |
| 18 | 57 | Benny Bloss | 7.829 | 1:26.18 |
| 19 | 86 | Mitchell Harrison | 8.575 | 1:26.92 |
| 20 | 122 | Justin Starling | 8.791 | 1:27.14 |
| 21 | 146 | Kevin Moranz | 9.271 | 1:27.62 |
| 22 | 33 | Fredrik Noren | 9.643 | 1:27.99 |