Daytona International Speedway - March 1, 2025
| Position | # | Rider | Interval | Best Time |
|---|---|---|---|---|
| 1 | 4 | Chase Sexton | 1:17.93 | |
| 2 | 32 | Justin Cooper | 0.121 | 1:18.05 |
| 3 | 21 | Jason Anderson | 1.040 | 1:18.97 |
| 4 | 94 | Ken Roczen | 1.104 | 1:19.04 |
| 5 | 2 | Cooper Webb | 1.350 | 1:19.28 |
| 6 | 46 | Justin Hill | 1.356 | 1:19.29 |
| 7 | 7 | Aaron Plessinger | 1.891 | 1:19.82 |
| 8 | 17 | Joey Savatgy | 1.903 | 1:19.84 |
| 9 | 51 | Justin Barcia | 2.296 | 1:20.23 |
| 10 | 14 | Dylan Ferrandis | 2.331 | 1:20.26 |
| 11 | 27 | Malcolm Stewart | 2.468 | 1:20.40 |
| 12 | 12 | Shane McElrath | 2.729 | 1:20.66 |
| 13 | 49 | Mitchell Oldenburg | 2.928 | 1:20.86 |
| 14 | 45 | Colt Nichols | 3.064 | 1:21.00 |
| 15 | 11 | Kyle Chisholm | 4.854 | 1:22.79 |
| 16 | 57 | Benny Bloss | 4.972 | 1:22.91 |
| 17 | 77 | Jerry Robin | 5.148 | 1:23.08 |
| 18 | 122 | Justin Starling | 5.602 | 1:23.53 |
| 19 | 72 | Anthony Rodriguez | 5.664 | 1:23.60 |
| 20 | 86 | Mitchell Harrison | 6.710 | 1:24.64 |
| 21 | 146 | Kevin Moranz | 6.942 | 1:24.88 |
| 22 | 33 | Fredrik Noren | 7.691 | 1:25.62 |